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Image Search Results
Journal: Microorganisms
Article Title: Spring Viremia of Carp Virus Infection Induces Carp IL-10 Expression, Both In Vitro and In Vivo
doi: 10.3390/microorganisms11112812
Figure Lengend Snippet: JAK-STAT, NF-κB and p38 signaling pathways played important roles in SVCV-induced carp IL-10 production. EPC cells were infected or uninfected with SVCV at MOI of 0.5, followed by treatment with JAK inhibitor CP-690550 ( A , B ), STAT3 inhibitor STA-21 ( C , D ), NF-κB inhibitor BAY11-7082 ( E , F ), p38 inhibitor SB202190 ( G , H ), JNK inhibitor SP600125 ( I , J ) or DMSO for 6 h, 12 h, 24 h and 48 h post-infection. The expression of carp IL-10 mRNA and protein were analyzed using RT-PCR and ELISA, respectively. Data are presented as the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 and, ns indicates no significant difference p > 0.05 compared with DMSO-treated plus SVCV-infected cells.
Article Snippet: JAK inhibitor (CP-690550), STAT3 inhibitor (STA-21),
Techniques: Infection, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay
Journal: Microorganisms
Article Title: Spring Viremia of Carp Virus Infection Induces Carp IL-10 Expression, Both In Vitro and In Vivo
doi: 10.3390/microorganisms11112812
Figure Lengend Snippet: ( A – F ): Gene expressions of JAK1, JAK2, JAK3, TYK2, STAT5 and STAT6 were analyzed using RT-PCR in EPC cells. EPC cells were infected or uninfected with SVCV at MOI of 0.5, followed by treatment with CP-690550, STA-21, BAY11-7082, SB202190, SP600125 and DMSO, respectively, at 6 h, 12 h, 24 h and 48 h post-infection. The gene expressions were analyzed using RT-PCR. Data are presented as the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 compared with DMSO-treated plus SVCV-infected cells.
Article Snippet: JAK inhibitor (CP-690550), STAT3 inhibitor (STA-21),
Techniques: Reverse Transcription Polymerase Chain Reaction, Infection